Where is the selection box when this happens? I see this on my scanner when the selection is not on a negative.
The holder has to be properly inserted into the registration holes on the right side of the scanner. The back of the holder has an area that must be kept clear of obstruction. About the whole width of the holder, and 1/2 inch of the glass should be showing. Assuming you are using the standard holders.
That doesn't look like the film holder that comes with the scanner - to my eyes. If it's not, you may have to use the 8X10 mask and select "Film area guide", then place your film holder on the glass. I'm grasping at straws here, based on what I see.
Things for you to check:
1. How are you setting the marquee to outline each picture? It doesn't seem you selected them. You should be able to use Thumbnail for auto marquee selection rather than Normal (manual selection) if you set point #2 correctly.
2. It appears you're scanning medium format. You have to select which medium format size you're scanning: 6x6, 6x7, etc. on the Configuration page. See this button on the bottom of your picture. When you get to the Configuration page, there's another tab for medium for that allows you to set the film size.
3. follow Jon's advice above.
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Because your unsharp mask button is checked, I'd bet money it has simply lost it's settings. Perhaps you'd saved them and can recall them?
Looks like you're blocking the calibration area at the top of the glass. Pull the holder down a half inch and try again.
I agree with Robert. It looks like something could be blocking the calibration area at the top of the bed.
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