I would invest in the Schneider 3x loupe designed for 6x7cm. It will allow you to critically evaluate your chromes and weed out soft images.The conundrum is that I have to examine my transparencies with my scanner.
Try to avoid f/45 and f/64. In my experience I try not to shoot past f/32.3 (3mm focus spread). It's also important to characterize your lenses to see what you can get away with. A simple high frequency subject imaged at f/22, f/32, f/45, and f/64 will quickly reveal a deterioration in image quality. For example, I know my 135, 210, and 300 APO Sironar-S lenses can handle f/45 if absolutely necessary, and still provide excellent image quality. However, f/45 is unacceptable on the 75 and 90 Grandagon-N's, so I will optimize my movements/composition to avoid the focus spreads requiring this aperture on the wide angles.
Have to agree with Ted on the sampling resolution having gone through this myself. All you are doing is accentuating film grain, thereby reducing your signal to noise ratio. The advantage of shooting 4x5 is that we can scan at lower resolution compared to the smaller formats for equivalent print size, thereby eliminating noise. If you are making exceptionally large images and therefore need to scan at maximum resolution for the pixel count, then you better plan on running a noise reduction program like Neat Image or Noise Ninja. But on a file that size, you can take a long break for lunch.
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