The only reliable way to minimize dust in film holders is to use a small hand vacuum, and vacuum film holders before loading them. After loading the holders, I keep my holders dust free in one gallon zip lock bags holding four holders -- others use a single holder in a bag. I vacuum the outside of the holder first, then remove one darkslide and vacuum the back of it, the inside of the holder, especially under the rails and flap. Then, I insert the darkslide and repeat on the other side.
In the field, before inserting the film holder in the camera, I make sure there aren't any dust particles or fibers on the dark slide, and if necessary wipe off any dust with a damp finger.
These precautions have made my films as close to dust free as possible. Using compressed canned air to blow the dust away just suspends the dust in the air, and it's gonna come down somewhere.
Lastly, I do occasionally have to spot the negative, I use Kodak Opaque Black (a discontinued product, I believe, but you can occasionally find someone selling some -- there are other products you can use, also) slightly diluted with distilled water. I use the finest brush I have (00000), and spot the base side of the negative. If you screw up, you can remove this stuff with a damp Q-tip. I also use a magnifying "Opti-visor" to see the small dust spots...
John Clark
www.johndclark.com
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